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numb overexpression plasmids  (OriGene)


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    Structured Review

    OriGene numb overexpression plasmids
    a Cell proliferation assay was performed using RealTime-Glo MT Cell Viability Assay (Promega) in <t>NUMB-overexpressing</t> (NUMB-wild type; S413A-non-phosphorylatable; S413D-phosphomimetic) melanoma cells till 72 h. b Cell migration was analyzed by wound healing assay in NUMB-modulated melanoma cells at 0, 24 and 48 h post-wound creation. c Cell invasion was analyzed by Matrigel invasion chambers using NUMB-modulated melanoma cells at 24 h. The quantitative data are presented as mean ± SEM with statistical significance compared to empty vector (pCMV6) control (** p < 0.01; *** p < 0.001; **** p < 0.0001) or compared to NUMB <t>overexpression</t> ( # p < 0.05; ### p < 0.001; #### p < 0.0001 in ≥2 biological replicates with ≥3 technical replicates and 2-3 images per replicate. Scale Bar=400 µm. Statistical significance was determined using one- or two-way ANOVA followed by Fisher’s LSD tests.
    Numb Overexpression Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/numb+overexpression+plasmids/NUMB+(NM_001005743)+Human+Tagged+ORF+Clone/pmc10771520-199-5-11
    Average 92 stars, based on 4 article reviews
    numb overexpression plasmids - by Bioz Stars, 2026-10
    92/100 stars

    Images

    1) Product Images from "Role of PLK1/NUMB/NOTCH in epithelial-mesenchymal transition in human melanoma"

    Article Title: Role of PLK1/NUMB/NOTCH in epithelial-mesenchymal transition in human melanoma

    Journal: NPJ Precision Oncology

    doi: 10.1038/s41698-023-00493-7

    a Cell proliferation assay was performed using RealTime-Glo MT Cell Viability Assay (Promega) in NUMB-overexpressing (NUMB-wild type; S413A-non-phosphorylatable; S413D-phosphomimetic) melanoma cells till 72 h. b Cell migration was analyzed by wound healing assay in NUMB-modulated melanoma cells at 0, 24 and 48 h post-wound creation. c Cell invasion was analyzed by Matrigel invasion chambers using NUMB-modulated melanoma cells at 24 h. The quantitative data are presented as mean ± SEM with statistical significance compared to empty vector (pCMV6) control (** p < 0.01; *** p < 0.001; **** p < 0.0001) or compared to NUMB overexpression ( # p < 0.05; ### p < 0.001; #### p < 0.0001 in ≥2 biological replicates with ≥3 technical replicates and 2-3 images per replicate. Scale Bar=400 µm. Statistical significance was determined using one- or two-way ANOVA followed by Fisher’s LSD tests.
    Figure Legend Snippet: a Cell proliferation assay was performed using RealTime-Glo MT Cell Viability Assay (Promega) in NUMB-overexpressing (NUMB-wild type; S413A-non-phosphorylatable; S413D-phosphomimetic) melanoma cells till 72 h. b Cell migration was analyzed by wound healing assay in NUMB-modulated melanoma cells at 0, 24 and 48 h post-wound creation. c Cell invasion was analyzed by Matrigel invasion chambers using NUMB-modulated melanoma cells at 24 h. The quantitative data are presented as mean ± SEM with statistical significance compared to empty vector (pCMV6) control (** p < 0.01; *** p < 0.001; **** p < 0.0001) or compared to NUMB overexpression ( # p < 0.05; ### p < 0.001; #### p < 0.0001 in ≥2 biological replicates with ≥3 technical replicates and 2-3 images per replicate. Scale Bar=400 µm. Statistical significance was determined using one- or two-way ANOVA followed by Fisher’s LSD tests.

    Techniques Used: Proliferation Assay, Viability Assay, Migration, Wound Healing Assay, Plasmid Preparation, Control, Over Expression

    Related Articles

    Over Expression:

    Article Title: Role of PLK1/NUMB/NOTCH in epithelial-mesenchymal transition in human melanoma.
    Article Snippet: Stable polyclonal pools were created for use by selection with 2 μg/ml of puromycin. pcDNA plasmids containing WT, K82R, or T210D PLK1 sequences were a kind gift from Dr. Mark Burkard, UW-Madison. .. Empty vector (pCMV6-Entry #PS100001) and NUMB overexpression plasmids were purchased from Origene (WT NUMB #RC220960 with custom mutated plasmids containing S413A and S413D mutations). ..

    Article Title: Role of PLK1/NUMB/NOTCH in epithelial-mesenchymal transition in human melanoma
    Article Snippet: Stable polyclonal pools were created for use by selection with 2 μg/ml of puromycin. pcDNA plasmids containing WT, K82R, or T210D PLK1 sequences were a kind gift from Dr. Mark Burkard, UW-Madison. .. Empty vector (pCMV6-Entry #PS100001) and NUMB overexpression plasmids were purchased from Origene (WT NUMB #RC220960 with custom mutated plasmids containing S413A and S413D mutations). ..



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    numb overexpression plasmids  (OriGene)
    92
    OriGene numb overexpression plasmids
    a Cell proliferation assay was performed using RealTime-Glo MT Cell Viability Assay (Promega) in <t>NUMB-overexpressing</t> (NUMB-wild type; S413A-non-phosphorylatable; S413D-phosphomimetic) melanoma cells till 72 h. b Cell migration was analyzed by wound healing assay in NUMB-modulated melanoma cells at 0, 24 and 48 h post-wound creation. c Cell invasion was analyzed by Matrigel invasion chambers using NUMB-modulated melanoma cells at 24 h. The quantitative data are presented as mean ± SEM with statistical significance compared to empty vector (pCMV6) control (** p < 0.01; *** p < 0.001; **** p < 0.0001) or compared to NUMB <t>overexpression</t> ( # p < 0.05; ### p < 0.001; #### p < 0.0001 in ≥2 biological replicates with ≥3 technical replicates and 2-3 images per replicate. Scale Bar=400 µm. Statistical significance was determined using one- or two-way ANOVA followed by Fisher’s LSD tests.
    Numb Overexpression Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/numb+overexpression+plasmids/NUMB+(NM_001005743)+Human+Tagged+ORF+Clone/pmc10771520-199-5-11
    Average 92 stars, based on 1 article reviews
    numb overexpression plasmids - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    a Cell proliferation assay was performed using RealTime-Glo MT Cell Viability Assay (Promega) in NUMB-overexpressing (NUMB-wild type; S413A-non-phosphorylatable; S413D-phosphomimetic) melanoma cells till 72 h. b Cell migration was analyzed by wound healing assay in NUMB-modulated melanoma cells at 0, 24 and 48 h post-wound creation. c Cell invasion was analyzed by Matrigel invasion chambers using NUMB-modulated melanoma cells at 24 h. The quantitative data are presented as mean ± SEM with statistical significance compared to empty vector (pCMV6) control (** p < 0.01; *** p < 0.001; **** p < 0.0001) or compared to NUMB overexpression ( # p < 0.05; ### p < 0.001; #### p < 0.0001 in ≥2 biological replicates with ≥3 technical replicates and 2-3 images per replicate. Scale Bar=400 µm. Statistical significance was determined using one- or two-way ANOVA followed by Fisher’s LSD tests.

    Journal: NPJ Precision Oncology

    Article Title: Role of PLK1/NUMB/NOTCH in epithelial-mesenchymal transition in human melanoma

    doi: 10.1038/s41698-023-00493-7

    Figure Lengend Snippet: a Cell proliferation assay was performed using RealTime-Glo MT Cell Viability Assay (Promega) in NUMB-overexpressing (NUMB-wild type; S413A-non-phosphorylatable; S413D-phosphomimetic) melanoma cells till 72 h. b Cell migration was analyzed by wound healing assay in NUMB-modulated melanoma cells at 0, 24 and 48 h post-wound creation. c Cell invasion was analyzed by Matrigel invasion chambers using NUMB-modulated melanoma cells at 24 h. The quantitative data are presented as mean ± SEM with statistical significance compared to empty vector (pCMV6) control (** p < 0.01; *** p < 0.001; **** p < 0.0001) or compared to NUMB overexpression ( # p < 0.05; ### p < 0.001; #### p < 0.0001 in ≥2 biological replicates with ≥3 technical replicates and 2-3 images per replicate. Scale Bar=400 µm. Statistical significance was determined using one- or two-way ANOVA followed by Fisher’s LSD tests.

    Article Snippet: Empty vector (pCMV6-Entry #PS100001) and NUMB overexpression plasmids were purchased from Origene (WT NUMB #RC220960 with custom mutated plasmids containing S413A and S413D mutations).

    Techniques: Proliferation Assay, Viability Assay, Migration, Wound Healing Assay, Plasmid Preparation, Control, Over Expression